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Microfluidic devices for circulating tumor cell capture
Funding: NYSTAR, NIH Clinical and Translational Science Center, Prostate Cancer Foundation

Rare cell capture from blood is exciting owing to the potential to derive clinical benefit with minimal inconvenience and discomfort to patients. Information derived from rare cells (e.g., fetal cells in mothers or cancer cells in cancer patients) can be used in lieu of information from biopsies and thus improve patient outcomes.

Microfluidic devices are ideally suited for these processes, owing to the flexibility of geometric design, wealth of chemical manipulation techniques, and assay compatibility of current systems. Stokes flow analysis is often a good predictor of the flows in these systems.

Our current work, in collaboration with Neil Bander, Evi Giannakakou, and David Nanus at Weill Cornell Medical Center, is focused on microfluidic capture of circulating tumor cells from prostate cancer patients with a view towards preclinical evaluation of chemotherapeutic efficacy.

A Transfer Function Approach for Predicting Rare Cell Capture Microdevice Performance
Archival Publications

Smith JP, Kirby BJ,
"A Transfer Function Approach for Predicting Rare Cell Capture Microdevice Performance", Biomedical Microdevices, submitted, 2015.

PDF version of Smith, Huang, Kirby:
Enhancing sensitivity and specificity in rare cell capture 
microdevices with dielectrophoresis

Smith JP, Huang C, Kirby BJ,
"Enhancing sensitivity and specificity in rare cell capture microdevices with dielectrophoresis", Biomicrofluidics, 9:014116, 2015. doi pdf

Saha T, Tsai S, Manning S, Thege FI, Voong C, Domchek S, Kirby BJ, Rhim AD
"Detection of circulating epithelial cells in BRCA mutation carriers without the diagnosis of cancer", submitted, 2014.

PDF version of Santana, Antonyak, Cerione, Kirby:
Microfluidic isolation of cancer-cell-derived microvesicles from heterogeneous extracellular shed vesicle populations

Santana SM, Antonyak MA, Cerione RA, Kirby BJ,
"Microfluidic isolation of cancer-cell-derived microvesicles from heterogeneous extracellular shed vesicle populations ", Biomedical Microdevices, 16:869–877, 2014. doi pdf

PDF version of Huang, Smith, Saha, Rhim, and Kirby:
Characterization of microfluidic shear-dependent EpCAM immunocapture and enrichment of pancreatic cancer cells from blood cells with dielectrophoresis

Huang C, Smith JP, Saha TM Rhim AD Kirby BJ
"Characterization of microfluidic shear-dependent EpCAM immunocapture and enrichment of pancreatic cancer cells from blood cells with dielectrophoresis", Biomicrofluidics, accepted, 2014. doi pdf

PDF version of Santana, Antonyak, Cerione, Kirby:
Cancerous Epithelial Cell Lines Shed Extracellular Vesicles With a Bimodal Size Distribution that is Sensitive to Glutamine Inhibition

Santana SM, Antonyak MA, Cerione RA, Kirby BJ,
"Cancerous Epithelial Cell Lines Shed Extracellular Vesicles With a Bimodal Size Distribution that is Sensitive to Glutamine Inhibition ", Physical Biology, 11:065001, 2014. doi pdf

PDF version of Rhim, Thege, Santana, Lannin, Kochman, Ginsberg, Lieb, Chandrasekhar, Drebin, Ahmad, Yang, Kirby, Stanger:
Detection of Circulating Pancreas Epithelial Cells in Patients With Pancreatic Cystic Lesions

Rhim AD, Thege FI, Santana SM, Lannin TB, Kochman ML, Ginsberg GG, Lieb JG, Chandrasekhar V, Drebin JA, Ahmad N, Yang Y-X, Kirby BJ, Stanger BZ
"Detection of Circulating Pancreas Epithelial Cells in Patients With Pancreatic Cystic Lesions", Gastroenterology 146:647:651, 2014. doi pdf

PDF version of Pratt, Stepansky, Hicks, Kirby:
Copy Number Analysis of Prostate Cancer Cells using GEDI Microdevices

Pratt ED, Stepansky A, Hicks J, Kirby BJ,
"Copy Number Analysis of Prostate Cancer Cells using GEDI Microdevices ", Analytical Chemistry, 86:11013-11017, 2014. doi pdf

PDF version of Galletti, Portella, Tagawa, Kirby, Giannakakou, Nanus:
Circulating tumor cells in prostate cancer diagnosis and monitoring: An appraisal of clinical potential

Galletti G, Portella L, Tagawa ST, Kirby BJ, Giannakakou PA, Nanus DM,
"Circulating tumor cells in prostate cancer diagnosis and monitoring: An appraisal of clinical potential ", Molecular Diagnostics and Therapy 2014. doi pdf

PDF version of Thege, Lannin, Saha, Tsai, Kochman, Hollingsworth, Rhim, Kirby:
Microfluidic immunocapture of circulating pancreatic cells with
parallel EpCAM and MUC1 capture modes

Thege FI, Lannin TB, Saha T, Tsai S, Kochman ML, Hollingsworth MA, Rhim AD, Kirby BJ,
"Microfluidic immunocapture of circulating pancreatic cells with parallel EpCAM and MUC1 capture modes", Lab Chip, 14:1775, 2014. doi pdf

PDF version of Smith, Lannin, Syed, Santana, Kirby:
Parametric control of collision rates and capture rates in geometrically enhanced
differential immunocapture (GEDI) microfluidic devices for rare cell capture

Smith JP, Lannin TB, Syed YA, Santana SM, Kirby BJ,
"Parametric control of collision rates and capture rates in geometrically enhanced differential immunocapture (GEDI) microfluidic devices for rare cell capture", Biomedical Microdevices, 16(1):143-151, 2014. doi pdf

PDF version of Galletti, Vahdat, Shah, Santana, Altavilla, Kirby, Giannakakou:
Isolation of breast cancer and gastric cancer circulating tumor cells by use of an anti HER2-based microfluidic device

Galletti G, Vahdat LT, Shah MA, Santana SM, Altavilla G, Kirby BJ, Giannakakou PA,
"Isolation of breast cancer and gastric cancer circulating tumor cells by use of an anti HER2-based microfluidic device ", Lab on a Chip, 14:147-156, 2014. doi pdf

PDF version of Huang, Santana, Liu, Bander, Hawkins, Kirby:
Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture

Huang C, Santana SM, Liu H, Bander NH, Hawkins BG, Kirby BJ
"Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture", Electrophoresis, 34:2970–2979, 2013. doi pdf

PDF version of Huang, Santana, Liu, Bander, Hawkins, Kirby:
Enrichment of prostate cancer cells from blood cells with a hybrid dielectrophoresis and immunocapture microfluidic system

Huang C, Liu H, Bander NH, Kirby BJ
"Enrichment of prostate cancer cells from blood cells with a hybrid dielectrophoresis and immunocapture microfluidic system", Biomedical Microdevices, 15(6):941-948, 2013. doi pdf

PDF version of Gleghorn, Smith, Kirby
Transport and collision dynamics in periodic asymmetric obstacle arrays:
rational design of micro
fluidic rare cell immunocapture devices

Gleghorn JP, Smith JP, Kirby BJ
"Transport and collision dynamics in periodic asymmetric obstacle arrays: rational design of micro fluidic rare cell immunocapture devices", Physical Review E, 88, 032136 (2013). doi pdf

PDF version of Zhu, Smith, Yarmush, Nahmias, Kirby, Murthy:
Microfluidic Enrichment of Mouse Epidermal Stem Cells and Validation of Stem Cell Proliferation In Vitro

Zhu B, Smith JP, Yarmush M, Nahmias Y, Kirby BJ, Murthy S,
"Microfluidic Enrichment of Mouse Epidermal Stem Cells and Validation of Stem Cell Proliferation In Vitro, Tissue Engineering C, 19(10):765-773, 2013. doi pdf

PDF version of Smith, Barbati, Santana, Gleghorn, Kirby:
Microfluidic transport in microdevices for rare cell capture

Smith JP, Barbati AC, Santana SM, Gleghorn JP, Kirby BJ,
"Microfluidic Transport in Microdevices for Rare Cell Capture", Electrophoresis, 33:3133-3142, 2012. doi pdf

PDF version of Diamond, Lee, Akhtar, Kirby, Giannakakou, Nanus:
Isolation and characterization of circulating tumor cells in prostate cancer

Diamond E, Lee GY, Akhtar NH, Kirby BJ, Giannakakou PA, Tagawa ST, Nanus DM,
"Isolation and characterization of circulating tumor cells in prostate cancer", Frontiers in Cancer Molecular Targets and Therapeutics, 2:131, 2012. doi pdf

PDF version of Kirby, Jodari, Loftus, Gakhar, Pratt, Chanel-Vos, Gleghorn, Santana, Liu, Smith, Navarro, Tagawa, Bander, Nanus, Giannakakou, Kirby:
Functional Characterization of Circulating Tumor Cells with a Prostate-Cancer-Specific Microfluidic Device

Kirby BJ, Jodari M, Loftus M, Gakhar G, Pratt ED, Chanel-Vos C, Gleghorn JP, Santana SM, Liu H, Smith JP, Navarro VN, Tagawa ST, Bander NH, Nanus DM, Giannakakou PA,
"Functional Characterization of Circulating Tumor Cells with a Prostate-Cancer-Specific Microfluidic Device ", PLoS ONE, 7(4): e35976, 2012. doi pdf

PDF version of authors title

Santana SM, Liu H, Bander NH, Gleghorn JP, Kirby BJ,
"Immunocapture of Prostate Cancer Cells with Anti-PSMA Antibodies in Microdevices", Biomedical Microdevices, Volume 14, Number 2, Pages 401-407, 2012. doi pdf

PDF version of AUTHORS: TITLE

Pratt ED, Huang C, Hawkins BG, Gleghorn JP, Kirby BJ
"Rare cell capture in microfluidic devices, Chemical Engineering Science, Vol 66(7)1508-1522, 2011. doi PubMedCentral pdf

PDF version of Gleghorn, Pratt, Denning, Liu, Bander, Tagawa, Nanus, Giannakakou, Kirby:
Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture (GEDI) and a prostate-specific antibody

Gleghorn JP, Pratt ED, Denning D, Liu H, Bander NH, Tagawa S, Nanus DM, Giannakakou PA, Kirby BJ
"Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture and a prostate-specific antibody", Lab on a Chip, 10:27-29, 2010. doi PubMedCentral pdf

Presentations and Other Publications
16-18 Feb 2015

Kirby BJ
5th Annual Circulating Tumor Cells and Cell-Free DNA conference, Molecular Medicine Tri-Conference, San Francisco, CA.

16-21 Nov 2014

Kirby BJ
AES Electrophoresis Society 2014 Meeting, AIChE 2014 Annual Meeting, Atlanta, GA.

26-30 October 2014

Thege FI , Kirby BJ
Parallel capture of pancreatic cancer cells, uTAS 2014, San Antonio, TX.

22-25 Oct 2014

Godla ME , Kirby BJ
Using Rare Cell Capture to Understand Metastasis, BMES 2014, San Antonio, TX.

3-8 August 2014

Pratt ED , Stepansky A , Hicks J , Kirby BJ
Single-nucleus sequencing of prostate cancer cells captured with GEDI microdevices, Gordon Research Conference on Rare Cells in Circulation, South Hadley, MA.

3-8 August 2014

Hartman J , Huang C , Kirby BJ
Hybrid immunocapture of ovarian cancer cells, Gordon Research Conference on Rare Cells in Circulation, South Hadley, MA.

3-8 August 2014

Thege FI , Kirby BJ
Parallel capture of pancreatic cancer cells, Gordon Research Conference on Rare Cells in Circulation, South Hadley, MA.

5-11 July 2014

Pratt ED , Stepansky A , Hicks J , Kirby BJ
Single-nucleus sequency of prostate cancer cells captured with GEDI microdevices, Gordon Research Conference on Genomic Instability, Hong Kong, CN.

21-22 June 2014

Lannin TB
Electrorotation of Cancer Cells to Optimize Performance of Dielectrophoresis Capture Devices, Gordon Research Conference on Bioanalytical Sensors, Newport, RI.

15-21 June 2014

Godla ME
Using Rare Cell Capture to Understand Metastasis, 13th International Summer School on Biocomplexity, Biodesign, and Bioinnovation, Istanbul, Turkey.

16-20 Mar 2014

Bono MS , Ahner BA , Kirby BJ
Multiplex measurement of algal lipid content via dielectric spectroscopy, flow cytometry, and 1H NMR, Division of Analytical Chemistry oral session on Analytical Chemistry in Energy, 2014 ACS National Meeting, Dallas, Texas.

7 Mar 2014

Lannin TB
Electrorotation of Cancer Cells to Optimize Performance of Dielectrophoresis Capture Devices, Sibley Graduate Research Symposium, Ithaca, NY.

13 Mar 2014

Kirby BJ
Cornell Materials Science Colloquium, Ithaca, NY.

18-22 Jan 2014

Smith JP , Gleghorn JP , Godla ME , Huang C , Lannin TB , Pratt ED , Santana SM , Thege FI , Kirby BJ
Circulating tumor cell (CTC) cancer biomarkers using geometrically enhanced differential immunocapture (GEDI) microdevices, Society for Lab Automation and Screening 2014, San Diego, CA

17 Jan 2014

Kirby BJ
Geometrically Enhanced Differential Immunocapture: Using obstacle arrays in microfluidic devices to enhance efficient and pure rare cell capture from fluid suspensions, Edwards Lecture, Oregon State University, Corvallis, OR.

7-11 Jan 2014

Santana SM , Fischbach-Teschl C , Kirby BJ ,
On-chip Tumor Microenvironment for Circulating Tumor Cell Isolation and Culture, Biomedical Engineering Society - Cellular and Molecular Bioengineering Meeting 2014, La Jolla, CA.

24-26 Nov 2013

Barbati AC , Kirby BJ
Streaming potential and conductivity measurements reveal electrokinetic properties of porous and charged layers, 66th Annual Meeting of the APS Division of Fluid Dynamics, November 24–26, 2013; Pittsburgh, Pennsylvania.

27-31 Oct 2013

Thege FI , Santana SM , Lannin TB , Tsai S , Saha TN , Godla ME , Pratt ED , Rhim AD , Kirby BJ
Parallelized Microfluidic Immunocapture of Circulating Pancreatic Cells for Genetic Analysis and Early Detection of Pancreatic Carcinogenesis MicroTAS 2013, Freiburg, Germany.

27-31 Oct 2013

Santana SM , Antonyak MA , Fischbach-Teschl C , Cerione RA , Kirby BJ
Novel Microfluidic Platforms for the Interrogation of Patient-Derived CTCs and Tumor-Derived Microvesicles MicroTAS 2013, Freiburg, Germany.

27-31 Oct 2013

Huang C , Smith JP , Liu H , Bander NH , Kirby BJ
Enrichment of prostate cancer cells from blood cells with a hybrid dielectrophoresis and immunocapture microfluidic system MicroTAS 2013, Freiburg, Germany.

25-28 Sep 2013

Huang C , Smith JP , Liu H , Bander NH , Kirby BJ
A Hybrid Dielectrophoresis and Immunocapture System for Enhanced Capture of Circulating Tumor Cells BMES 2013, Seattle, WA.

25-28 Sep 2013

Lannin TB , Sung MS , Thege FI , Syed YA , Galletti GI , Rhim AD , Giannakakou PA , Kirby BJ
Machine Learning Outperforms Manual Counting for Classifying Circulating Tumor Cells from Cancer Patients BMES 2013, Seattle, WA.

25-28 Sep 2013

Huang C , Santana SM , Antonyak MA , Fischbach-Teschl C , Cerione RA , Kirby BJ
Novel Micro?uidic Platforms for the Interrogation of Patient-Derived CTCs and Tumor-Derived Microvesicles BMES 2013, Seattle, WA.

25-28 Sep 2013

Thege FI , Santana SM , Rhim AD , Kirby BJ
Multiplexed Micro?uidic Immunocapture of Circulating Pancreas Cells for the Early Detection of Pancreatic Carcinogenesis BMES 2013, Seattle, WA.

19 Sep 2013

Huang C , Smith JP , Kirby BJ
A Hybrid Dielectrophoresis and Immunocapture Microfluidic System for Enhanced Rare Cell Capture Cornell NanoScale Science & Technology Facility Annual Meeting, Ithaca, NY.

28-31 Jul 2013

Kirby BJ
Electrokinetic response in systems with fluid-permeable and charged layers, Society of Engineering Science ASME-AMD Summer Meeing, 2013, Providence, RI.

9-14 Jun 2013

Thege FI , Santana SM , Rhim AD , Kirby BJ
Multiplexed Micro?uidic Immunocapture of Circulating Pancreas Cells for the Early Detection of Pancreatic Carcinogenesis Gordon Research Conference on the Physics and Chemistry of Microfluidics, 2013, Lucca, Italy.

9-14 Jun 2013

Lannin TB , Sung MS , Thege FI , Syed YA , Galletti GI , Rhim AD , Giannakakou PA , Kirby BJ
Machine Learning Outperforms Manual Counting for Classifying Circulating Tumor Cells from Cancer Patients Gordon Research Conference on the Physics and Chemistry of Microfluidics, 2013, Lucca, Italy.

9-14 Jun 2013

Kirby BJ , Gleghorn JP , Godla ME , Huang C , Lannin TB , Pratt ED , Santana SM , Smith JP , Syed YA , Thege FI
Capture and interrogation of circulating tumors cells by use of geometrically enhanced differential immunocapture (GEDI) microfluidic devices Gordon Research Conference on the Physics and Chemistry of Microfluidics, 2013, Lucca, Italy.

6-7 Jun 2013

Kirby BJ
Select Biosciences 2013 Nano and Microfluidics conference, CCH Congress Centre, Hamburg Germany.

31 May-4 Jun 2013

Antonarakis ES , Giannakakou PA , Kirby BJ , Nicasio LV , Eisenberger MA , Nanus DM , Tagawa ST
TAXYNERGY (NCT01718353): A randomized phase II trial examining an early switch from first-line docetaxel to cabazitaxel, or cabazitaxel to docetaxel, in men with metastatic castration-resistant prostate cancer (mCRPC) ASCO 2013, Chicago, IL.

6-10 Apr 2013

Galletti GI , Kirby BJ , Vahdat LT , Giannakakou PA
Development of a HER2-based GEDI microfluidic device for the molecular characterization of CTCs from metastatic breast cancer patients AACR 2013, Washington, DC.

6-10 Apr 2013

Sung MS , Gyrezi A , Lee GY , Matov A , Galletti GI , Loftus M , Syed YA , Lannin TB , Hristov A , Mason C , Tagawa S , Kirby BJ , Nanus DM , Giannakakou PA
Using CTCs to interrogate mechanisms of taxane resistance in the prospective TAXYNERGY clinical trial in prostate cancer AACR 2013, Washington, DC.

17-21 Mar 2013

Kirby BJ
"Functional Characterization of Circulating Tumor Cells Captured With GEDI Microdevices", Pittcon 2013, Philadelphia, PA.

10-15 Feb 2013

Santana SM , Fischbach-Teschl C , Kirby BJ ,
Capture and Interrogation of Circulating Tumor Cells in Tissue-Engineered Geometrically Enhanced Differential Immunocapture Microfluidic Devices Gordon Research Conference on Fibronectin, Integrins, & Related Molecules, 2013, Ventura, CA.

12-16 Nov 2012

Pratt ED , Gleghorn JP , Loftus M , Jodari M , Santana SM , Smith JP , Stepansky A , Giannakakou PA , Hicks J , Nanus DM , Rhim AD , Stanger BZ , Kirby BJ
Functional and genetic analysis of CTCs captured with GEDI microdevices, World CTC Summit Boston 2012, Boston, MA.

7 Nov 2012

Kirby BJ
"Electrokinetic and immunospecific characterization of cells for human health and energy applications", SUNY-Buffalo Chemical and Biomolecular Engineering Seminar Series, Buffalo, NY.

24-27 Oct 2012

Pratt ED , Blattner M , Stepansky A , Liu H , Bander NH , Hicks J , Rubin MA , Kirby BJ
"Microfluidic Nuclei Extraction from Circulating Tumor Cells for Genetic Analyses", BMES 2012, Atlanta, Georgia.

24-27 Oct 2012

Huang C , Kirby BJ
"DEP-enhanced immunocapture of Circulating Tumor Cells in GEDI Microdevices", BMES 2012, Atlanta, Georgia.

24-27 Oct 2012

Santana SM , Fischbach C , Kirby BJ
"Immunocapture of Circulating Tumor Cells in Tissue-Engineered GEDI Microdevices", BMES 2012, Atlanta, Georgia.

24-27 Oct 2012

Kirby BJ
"Dielectric Spectrometry of Mycobacterium and GEDI Devices for Circulating Tumor Cells: Cell Capture, Characterization, and Manipulation in Geometrically Patterned Microstructures", BMES 2012, Atlanta, Georgia.

30 Sep-5 Oct 2012

Kirby BJ
"Dielectric and dielectrophoretic measurements and manipulations of cells for human health and energy applications", SciX 2012, Kansas City, MO.

19-21 Sep 2012

Kirby BJ
CHI ADAPT 2012, Washington, DC.

17 Aug 2012

Huang C , Santana SM , Hawkins BG , Liu H , Bander NH , Kirby BJ
"Dielectrophoresis-Enhanced Immunocapture of Prostate Cancer Cells", cornell BMES Retreat, Ithaca, NY.

10-13 May 2012

Kirby BJ
"Geometric patterning: controlling small-scale flows with repeated microstructures", German--American Frontiers of Science Symposium, Potsdam, Germany. | video

31 Mar-4 Apr 2012

Galletti G, , Kirby BJ , Vahdat L , Giannakakou PA
Development of a novel microfluidic device for the capture and molecular characterization of circulating tumor cells from metastatic breast cancer patients 103nd annual AACR meeting 2012, Chicago, IL.

31 Mar-4 Apr 2012

Sung M , Loftus M , Jodari-Karimi M , Pratt ED , Gakhar G , Rubin M , Bander N , Novaro , Tagawa ST , Kirby BJ , Nanus DM , Giannakakou PA
Molecular and functional analysis of circulating prostate tumor cells with anti-PSMA immunocapture 103nd annual AACR meeting 2012, Chicago, IL.

2-3 Feb 2012

Kirby BJ
Select Biosciences Meeting on Circulating Tumor Cells, San Diego, CA.

12-15 Oct 2011

Kirby BJ, Pratt ED, Santana SM, Smith JP, Gleghorn JP, Liu H, Bander NH, Jodari M, Loftus M, Nanus DM, Giannakakou PA,
"Functional Characterization of drug-target engagement on high-purity CTCs correlate with patient progression", BMES 2011, Hartford, CT.

2-6 Oct 2011

Kirby BJ, Pratt ED, Santana SM, Smith JP, Gleghorn JP, Jodari M, Gakhar G, Loftus M, Liu H, Bander NH, Nanus DM, Giannakakou PA,
"Functional Characterization of drug-target engagement on high-purity CTCs correlate with patient progression", MicroTAS 2011, Seattle, WA.

2-6 Oct 2011

Pratt ED, Santana SM, Gleghorn JP, Liu H, Bander NH, Nanus DM, Giannakakou PA, Kirby BJ
"Circulating tumor cell release by use of novel immunocapture chemistry in GEDI microdevices", MicroTAS 2011, Hartford, CT.

1 Oct 2011

Kirby BJ
Microfluidic capture of circulating tumor cells for assay of chemotherapeutic efficacy: system design and translation to the clinical setting Cornell Translational Cancer Research Symposium, Ithaca, NY

19-20 Apr 2011

Kirby BJ
NSF National Nanotechnology Infrastructure Network Computation Symposium, Ann Arbor, MI.

10-12 Apr 2011

Santana SM, Liu H, Bander NH, Gleghorn JP, Kirby BJ
"Immunocapture and Release of Prostate Circulating Tumor Cells with anti-PSMA Antibodies in Microfluidic Devices", PS-OC Network Investigators' Meeting, 2011, La Jolla, CA.

2-6 Apr 2011

Loftus M , Jodari-Karimi M , Pratt ED , Gakhar G , Rubin M , Bander N , Novaro , Tagawa ST , Kirby BJ , Nanus DM , Giannakakou PA
Molecular and functional analysis of circulating tumor cells in castrate resistant prostate cancer using a geometrically enhanced microfluidic device based on PSMA immunocapture, 102nd annual AACR meeting 2011, Orlando, FL.

1-3 Apr 2011

Santana SM, Liu H, Bander NH, Gleghorn JP, Kirby BJ
"Immunocapture and Release of Prostate Circulating Tumor Cells with anti-PSMA Antibodies in Microfluidic Devices", Northeast Bioengineering Conference, Troy, NY.

23-25 Feb 2011

Kirby BJ
CHI Meeting on Circulating Tumor Cells (CTCs) for Cancer Detection, Diagnosis, Prognosis and Treatment, San Francisco, CA.

17-19 Feb 2011

Jodari M , Loftus M , Gleghorn JP , Gakhar G , Pratt ED , Tagawa ST , Bander NH , Giannakakou PA , Kirby BJ , Nanus DM
"Capture and analysis of prostate cancer circulating tumor cells (CTCS) by use of geometrically enhanced differential immunocapture (GEDI)", 2011 Genitourinary Cancers Symposium, Orlando, FL.

7-11 Feb 2011

Kirby BJ
Lorentz workshop on circulating tumor cells, Leiden, The Netherlands.

12-18 Nov 2010

Smith JP , Gleghorn JP , Kirby BJ
"Particle collision dynamics of microparticles in microfluidic obstacle arrays", ASME 2010 International Mechanical Engineering Congress and Exposition, Vancouver, BC.

7-12 Nov 2010

Gleghorn JP , Smith JP , Kirby BJ
"Particle collision dynamics of microparticles in microfluidic obstacle arrays", AIChE 2010, Salt Lake City, UT.

6-9 Oct 2010

Gleghorn JP , Santana SM , Pratt ED , Loftus MS , Jodari-Karimi M , Bander NH , Nanus DM , Giannakakou PA , Kirby BJ
"Cancer cell assays by use of immunocapture, subcellular imaging, and cell release in GEDI microdevices", BMES 2010, Austin, TX.

3-7 Oct 2010

Gleghorn JP , Santana SM , Pratt ED , Loftus MS , Jodari-Karimi M , Bander NH , Nanus DM , Giannakakou PA , Kirby BJ
"Cancer cell assays by use of immunocapture, subcellular imaging, and cell release in GEDI microdevices", MicroTAS 2010, Groningen, The Netherlands.

3-7 Oct 2010

Gleghorn JP , Santana SM , Pratt ED , Loftus MS , Jodari-Karimi M , Bander NH , Nanus DM , Giannakakou PA , Kirby BJ
"Cancer cell assays by use of immunocapture, subcellular imaging, and cell release in GEDI microdevices", MicroTAS 2010, Groningen, The Netherlands.

3-7 Oct 2010

Huang C , Hawkins BG , Arisanipalai S , Kirby BJ
"Automated dielectrophoretic characterization for microfluidic cell separation devices", MicroTAS 2010, Groningen, The Netherlands.

22 Sep 2010

Kirby BJ
"Microfluidic capture and analysis of CTCs from peripheral blood of prostate cancer patients", Georgia Tech Chemical and Biomolecular Engineering Seminar, Atlanta, GA.

15 July 2010

Kirby BJ
"Microfluidic devices for capture and analysis of prostate cancer CTCs from peripheral blood samples", Memorial Sloan-Kettering Cancer Center, New York, NY.

20-25 June 2010

Pratt ED , Gleghorn JP , Santana SM , Loftus MS , Jodari-Karimi M , Bander NH , Nanus DM , Giannakakou PA , Kirby BJ
"Cancer cell assays by use of immunocapture, subcellular imaging, and cell release in GEDI microdevices", Gordon Research Conference on Bioanalytical Sensors, Colby-Sawyer College, New London, NH.

20-25 June 2010

Kirby BJ
"Microfluidic devices for capture and analysis of prostate cancer CTCs from peripheral blood samples", Gordon Research Conference on Bioanalytical Sensors, Colby-Sawyer College, New London, NH.

18 June 2010

Smith JP, Gleghorn JP, Kirby BJ
"Particle Collision Dynamics in Geometrically Enhanced Differential Immunocapture (GEDI) Microdevices", 10th New York Complex Matter Workshop, Ithaca, NY.

18 June 2010

Santana SM, Gleghorn JP, Kirby BJ
"Immunocapture and Release of Viable Circulating Tumor Cells in Microdevices", 10th New York Complex Matter Workshop, Ithaca, NY.

17-21 April 2010

Loftus MS , Gleghorn JP , Pratt ED , Bander NH , Nanus DM , Kirby BJ
Giannakakou P
"Analysis of circulating tumor cells from castrate resistant prostate cancer patients captured via a microfluidic device using an anti-PSMA antibody", American Association of Cancer Researchers 2010 Annual Meeting, Washington, DC.

17 Mar 2010

Santana SM, Gleghorn JP, Pratt ED, Bander NH, Nanus DM, Giannakakou PA, Kirby BJ
"Fluid Mechanics of Rare Cell Transport and Adhesion", Cornell Engineering Research Conference, Ithaca, NY.

1-5 Nov 2009 Gleghorn JP, Pratt ED, Loftus MS, Levy BE, Bander NH, Nanus DM, Giannakakou PA, Kirby BJ
"High efficiency capture of circulating tumor cells from patient blood using geometrically-enhanced differential immunocapture", MicroTAS 2009, Jeju, Korea.
27 Oct 2008

Kirby BJ
"Geometric patterning to control cell growth, capture, and transport", NBTC Annual Symposium, Ithaca, NY.

21 Mar 2008

Kirby BJ
"Cell processing in microdevices: sorting, capturing, and concentration techniques", University of Rochester Biomedical Engineering Department, Rochester, NY.

Comparison of collision frequencies of cells in straight and GEDI (refs here and here) obstacle arrays.
Sanger sequencing of the T868A androgen receptor mutation in cancer cells. Top: healthy blood sample shows wild-type androgen receptor. Middle: cancer cell culture shows T868A A-G single point mutation. Bottom: even at 50 cells/mL, cancer cells in blood can be captured by GEDI devices (refs here and here) at a purity such that the mutated form dominates the sequencing data.
Inside Cornell press event to discuss our 2012 PLoS ONE paper and TAXYNERGY clinical trial.
Charlie's work on dielectrophoretic trapping of cancer cells in a Hele-Shaw device. Here, data showing the benefits of combining DEP with immunocapture for LNCaP cells. (see ref here).
Collaborative work led by Shashi Murthy's group at Northeastern. Imaging of colony growth following microfluidic stem cell enrichment. (see ref here).