Cornell University - Visit www.cornell.edu Kirby Research Group at Cornell: Microfluidics and Nanofluidics : - Home College of Engineering - visit www.engr.cornell.edu Cornell University - Visit www.cornell.edu
Cornell University, College of Engineering Search Cornell
News Contact Info Login
Cellular Analysis
Lab-on-a-Chip devices provide an exciting opportunity to perform unique cellular analyses, due to the potential for:
  • processing of extraordinarily small volumes (comparable to cellular contents)
  • rapid automated analysis
  • analysis of single-cells
Our interests related to cellular analysis focus mainly on laser-manipulation and lysing of cells combined with microchip dialysis of cytosolic contents.

Selected Publications and Presentations on Cellular Analysis

Rouillard AD, Berglund CM, Lee JY, Polacheck WJ, Tsui YT, Bonassar LJ, Kirby BJ
"Methods for photocrosslinking alginate hydrogel scaffolds with high cell viability," Tissue Engineering C, accepted, 2010.

PDF version of Rouillard, Tsui, Polacheck, Lee, Bonassar, Kirby: 
Control of the Electromechanical Properties of
Alginate Hydrogels via Ionic and Covalent
Cross-Linking and Microparticle Doping

Rouillard AD, Tsui YT, Polacheck WJ, Lee JY, Bonassar LJ, Kirby BJ
"Control of the Electromechanical Properties of Alginate Hydrogels via Ionic and Covalent Crosslinking and Microparticle Doping," Biomacromolecules, 11 (8), pp 2184–2189, 2010. doi pdf

Hawkins BG, Kirby BJ
"Electrothermal flow effects in insulating (electrodeless) dielectrophoresis systems," submitted, 2010.

Pratt ED, Huang C, Hawkins BG, Gleghorn JP, Kirby BJ
"Rare cell capture in microfluidic devices, submitted, 2010.

Rouillard AD, Berglund CM, Lee JY, Polacheck WJ, Tsui YT, Bonassar LJ, Kirby BJ
"Methods for photocrosslinking alginate hydrogel scaffolds with high cell viability," Orthopedic Research Society Meeting, New Orleans, LA, March 2010.

PDF version of Gleghorn, Pratt, Denning, Liu, Bander, Tagawa, Nanus, Giannakakou, Kirby:
Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture (GEDI) and a prostate-specific antibody

Gleghorn JP, Pratt ED, Denning D, Liu H, Bander NH, Tagawa S, Nanus DM, Giannakakou PA, Kirby BJ
"Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture and a prostate-specific antibody", Lab on a Chip, 10:27-29, 2010. doi pdf

hawkins BG, Huang C, Stranic I, Kondapalli SK, Kirby BJ
"Particle screening in polymeric microfluidic devices using insulating dielectrophoresis and frequency modulated electric fields", ", MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.

Kondapalli SK, Connelly JT, Baeumner AJ, Kirby BJ
"On-chip electrophoretic concentration of liposomes for antibody-based viral biosensors", ", MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.

PDF version of Brian Kirby Nanofluidics review

Kirby BJ
"Nanofluidics" (book review), Materials Today, 12(5) 51, 2009. doi pdf

Hawkins BG, Tandon V, Kirby BJ
"Electrokinetic tools for cellular screening in plastic microdevices: interfacial characterization and engineering design", Meeting of the Electrochemical Society, Washington, DC, October 2007.

Rouillard AD, Tsui Y, Polacheck WJ, Lee JY, Bonassar LJ, Kirby BJ
"Control of the electromechanical proterties of alginate tissue scaffolds via ionic and covalent crosslinking and microparticle doping", BMES 2007, Los Angeles, CA, September 2007.

PDF version of Benjamin G. Hawkins, A. Ezekial Smith, 
Yusef A. Syed, Brian J. Kirby: 
Continuous-Flow Particle Separation 
by 3D Insulative Dielectrophoresis 
Using Coherently Shaped, dc-Biased, ac Electric Fields

Hawkins BG, Smith AE, Syed YA, Kirby BJ
"Continuous-flow particle separation by 3D insulative dielectrophoresis using coherently shaped, DC-biased, AC electric fields," Analytical Chemistry, 2007. doi pdf text

Rouillard AD, Bonassar LJ, Kirby BJ
"Control of electrokinetic properties of hydrogels for studies of mechanotransduction in chondrocytes," BMES Annual Meeting Chicago, IL, Oct 2006.

Rouillard AD, Bonassar LJ, Kirby BJ
"Studying cell mechanotransduction in chondrocytes via manipulation of electrokinetics in alginate hydrogels," 2nd New York Complex Matter Workshop Ithaca, NY, June 2006.

Wilkes JO , with Birmingham SG, Kirby BJ, Cheng C-Y
"Fluid Mechanics for Chemical Engineers with Microfluidics and CFD," Prentice-Hall, 2005.
click here to go to text webpage

PDF version of Reichmuth, Shepodd, Kirby: Microchip HPLC of peptides and proteins

Reichmuth DS, Shepodd TJ, Kirby BJ
"Microchip HPLC of peptides and proteins," Analytical Chemistry 77:2997-3000 (2005). doi pdf text

PDF version of Song, Mela, van den Berg, Kirby: Microfluidic architectures for integrated cell lysis, lysate dialysis and cell stimulus

Song S, Mela P, van den Berg A, Kirby BJ
"Microfluidic architectures for integrated cell lysis, lysate dialysis and cell stimulus," in MicroTAS 2004, Kluwer Academic Publishers (2004). pdf

PDF version of Kirby, Wheeler, Zare, Fruetel, Shepodd: Programmable modification of cell adhesion and zeta potential in silica microchips

Kirby BJ, Wheeler AR, Zare RN, Fruetel JA, Shepodd TJ
"Programmable Modification of Cell Adhesion and Zeta Potential in Silica Microchips,"Lab On a Chip 3:5-10 (2003). doi pdf text

PDF version of Kirby, Wheeler, Shepodd, Fruetel, Hasselbrink, Zare: A laser-polymerized thin film silica surface modification for suppression of cell adhesion and electroosmotic flow in microchannels

Kirby BJ, Wheeler AR, Shepodd TJ, Fruetel JA, Hasselbrink, Jr. EF, Zare RN
"A Laser-Polymerized Thin Film Silica Surface Modification for Suppression of Cell Adhesion and Electroosmotic Flow in Microchannels," MicroTAS 2001, Kluwer Academic Publishers, (2001). pdf

Microdevices for neuron culture.