|
Cellular Analysis
|
Lab-on-a-Chip devices provide an exciting opportunity to perform unique cellular
analyses, due to the potential for:
- processing of extraordinarily small
volumes (comparable to cellular contents)
- rapid automated analysis
- analysis of single-cells
Our interests related to cellular analysis focus mainly on laser-manipulation
and lysing of cells combined with microchip dialysis of cytosolic contents.
|
Selected
Publications and Presentations on Cellular Analysis
|
|
Rouillard AD,
Berglund CM,
Lee JY,
Polacheck WJ,
Tsui YT,
Bonassar LJ,
Kirby BJ
"Methods for photocrosslinking alginate hydrogel scaffolds with
high cell viability,"
submitted, 2009.
|
|
Gleghorn JP,
Pratt ED,
Denning D,
Liu H,
Bander NH,
Tagawa S,
Nanus DM,
Giannakakou PA,
Kirby BJ
"Capture of circulating tumor cells from whole blood of
prostate cancer patients using geometrically enhanced
differential immunocapture and a prostate-specific antibody",
Lab on a Chip, 2010.
doi
|
|
Rouillard AD,
Tsui YT,
Polacheck WJ,
Lee JY,
Bonassar LJ,
Kirby BJ
"Control of the Electromechanical Properties of Alginate Hydrogels via
Ionic and Covalent Crosslinking and Microparticle Doping,"
submitted, 2009.
|
 |
Kirby BJ
"Nanofluidics" (book review),
Materials Today, 12(5) 51, 2009.
doi
pdf
|
|
Hawkins BG,
Tandon V,
Kirby BJ
"Electrokinetic tools for cellular screening in plastic microdevices: interfacial
characterization and engineering design",
Meeting of the Electrochemical Society, Washington, DC, October 2007.
|
|
Hawkins BG,
Smith AE,
Kirby BJ
"High-throughput, continuous-flow, dielectrophoretic
screening of Mycobacterium smegmatis in
coherently patterned, polymeric microchannels",
MicroTAS 2007, Paris, France, October 2007.
|
|
Hawkins BG,
Smith AE,
Syed YA,
Kirby BJ
"Continuous-flow particle separation by 3D insulative
dielectrophoresis using coherently shaped, DC-biased,
AC electric fields,"
Analytical Chemistry, 2007.
doi
pdf
text
|
|
Hawkins BG,
Smith AE, Syed YA,
Kirby BJ
"Electrokinetic particle sorting in cyclic olefin polymer microsystems,"
AIChE 2006 San Francisco, CA, Nov 2006.
|
|
Smith AE,
Hawkins BG,
Syed YA,
Kirby BJ
"Continuous-flow particle sorting at low applied electric fields
using electrodeless dielectrophoresis in ridged polymeric devices,"
MicroTAS 2006 Tokyo, Japan, Nov 2006.
|
|
Rouillard AD,
Bonassar LJ,
Kirby BJ
"Control of electrokinetic properties of hydrogels for studies of mechanotransduction
in chondrocytes,"
BMES Annual Meeting Chicago, IL, Oct 2006.
|
|
Wilkes JO , with Birmingham SG,
Kirby BJ, Cheng C-Y
"Fluid Mechanics for Chemical Engineers with Microfluidics and CFD,"
Prentice-Hall, 2005.
click here to go to text webpage
|
|
Reichmuth DS, Shepodd TJ,
Kirby BJ
"Microchip HPLC of peptides and proteins,"
Analytical Chemistry 77:2997-3000 (2005).
doi
pdf
text
|
| |
Kirby BJ,
Wheeler AR, Zare RN, Fruetel JA,
Shepodd TJ
"Programmable Modification of Cell Adhesion
and Zeta Potential in Silica Microchips,"Lab On a Chip
3:5-10 (2003).
doi
pdf
text
|
|
|
|
Cartoon of cellular transport over ridged microchannels
that sort using
dielectrophoresis. Place mouse on image to animate.
Here, a high-DEP cell (red) is deflected as compared to a
low-DEP cell (green).
| |
|
