|
Cellular Analysis
|
Lab-on-a-Chip devices provide an exciting opportunity to perform unique cellular
analyses, due to the potential for:
- processing of extraordinarily small
volumes (comparable to cellular contents)
- rapid automated analysis
- analysis of single-cells
Our interests related to cellular analysis focus mainly on laser-manipulation
and lysing of cells combined with microchip dialysis of cytosolic contents.
|
Selected
Publications and Presentations on Cellular Analysis
|
|
Rouillard AD,
Berglund CM,
Lee JY,
Polacheck WJ,
Tsui YT,
Bonassar LJ,
Kirby BJ
"Methods for photocrosslinking alginate hydrogel scaffolds with
high cell viability,"
Tissue Engineering C, accepted, 2010.
|
|
Rouillard AD,
Tsui YT,
Polacheck WJ,
Lee JY,
Bonassar LJ,
Kirby BJ
"Control of the Electromechanical Properties of Alginate Hydrogels via
Ionic and Covalent Crosslinking and Microparticle Doping,"
Biomacromolecules, 11 (8), pp 2184–2189, 2010.
doi
pdf
|
|
Hawkins BG,
Kirby BJ
"Electrothermal flow effects in insulating
(electrodeless) dielectrophoresis systems,"
submitted, 2010.
|
|
Pratt ED,
Huang C,
Hawkins BG,
Gleghorn JP,
Kirby BJ
"Rare cell capture in microfluidic devices, submitted, 2010.
|
|
Rouillard AD,
Berglund CM,
Lee JY,
Polacheck WJ,
Tsui YT,
Bonassar LJ,
Kirby BJ
"Methods for photocrosslinking alginate hydrogel scaffolds with high cell viability,"
Orthopedic Research Society Meeting, New Orleans, LA, March 2010.
|
|
Gleghorn JP,
Pratt ED,
Denning D,
Liu H,
Bander NH,
Tagawa S,
Nanus DM,
Giannakakou PA,
Kirby BJ
"Capture of circulating tumor cells from whole blood of
prostate cancer patients using geometrically enhanced
differential immunocapture and a prostate-specific antibody",
Lab on a Chip, 10:27-29, 2010.
doi
pdf
|
|
hawkins BG,
Huang C,
Stranic I,
Kondapalli SK,
Kirby BJ
"Particle screening in polymeric microfluidic devices using insulating
dielectrophoresis and frequency modulated electric fields", ",
MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.
|
|
Kondapalli SK,
Connelly JT,
Baeumner AJ,
Kirby BJ
"On-chip electrophoretic concentration of liposomes
for antibody-based viral biosensors", ",
MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.
|
|
Kirby BJ
"Nanofluidics" (book review),
Materials Today, 12(5) 51, 2009.
doi
pdf
|
|
Hawkins BG,
Tandon V,
Kirby BJ
"Electrokinetic tools for cellular screening in plastic microdevices: interfacial
characterization and engineering design",
Meeting of the Electrochemical Society, Washington, DC, October 2007.
|
|
Rouillard AD,
Tsui Y,
Polacheck WJ,
Lee JY,
Bonassar LJ,
Kirby BJ
"Control of the electromechanical proterties of alginate
tissue scaffolds via ionic and covalent crosslinking
and microparticle doping", BMES 2007, Los Angeles, CA, September 2007.
|
|
Hawkins BG,
Smith AE,
Syed YA,
Kirby BJ
"Continuous-flow particle separation by 3D insulative
dielectrophoresis using coherently shaped, DC-biased,
AC electric fields,"
Analytical Chemistry, 2007.
doi
pdf
text
|
|
Rouillard AD,
Bonassar LJ,
Kirby BJ
"Control of electrokinetic properties of hydrogels for studies of mechanotransduction
in chondrocytes,"
BMES Annual Meeting Chicago, IL, Oct 2006.
|
|
Rouillard AD,
Bonassar LJ,
Kirby BJ
"Studying cell mechanotransduction in chondrocytes via manipulation of electrokinetics in
alginate hydrogels,"
2nd New York Complex Matter Workshop Ithaca, NY, June 2006.
|
|
Wilkes JO , with Birmingham SG,
Kirby BJ, Cheng C-Y
"Fluid Mechanics for Chemical Engineers with Microfluidics and CFD,"
Prentice-Hall, 2005.
click here to go to text webpage
|
|
Reichmuth DS, Shepodd TJ,
Kirby BJ
"Microchip HPLC of peptides and proteins,"
Analytical Chemistry 77:2997-3000 (2005).
doi
pdf
text
|
|
Song S, Mela P, van den Berg A,
Kirby BJ
"Microfluidic architectures for integrated cell lysis,
lysate dialysis and cell stimulus," in MicroTAS 2004, Kluwer Academic Publishers (2004).
pdf
|
|
Kirby BJ,
Wheeler AR, Zare RN, Fruetel JA,
Shepodd TJ "Programmable Modification of Cell Adhesion
and Zeta Potential in Silica Microchips,"Lab On a Chip
3:5-10 (2003).
doi
pdf
text
|
|
Kirby BJ,
Wheeler AR, Shepodd TJ, Fruetel JA,
Hasselbrink, Jr. EF, Zare RN "A Laser-Polymerized Thin
Film Silica Surface Modification for Suppression of Cell
Adhesion and Electroosmotic Flow in Microchannels,"
MicroTAS 2001, Kluwer Academic Publishers,
(2001).
pdf
|
|
|