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Microfluidics
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Microfluidic devices are characterized by channels with
diameters ranging roughly
between 100 nm and 100 microns, often involving particles
with diameters ranging
roughly from 10 nm to 10 microns. At these length scales,
the Reynolds number
is low and the flow is usually laminar, but the mass transfer Peclet
number is often large, leading to unique microfluidic mixing regimes. Because the diameters are
small and it is
difficult to generate large flow velocities with pressure,
other effects can
dominate. In particular, electrokinetic effects
(electroosmosis and
electrophoresis) can dominate and voltage can be
used to manipulate fluids,
molecules, and particles. A variety of chemical separations
have been developed in microfluidic
devices owing to the beneficial species transport
often associated with microchip devices.
Surface tension can also
be very important, and
bubbles and drops can often be manipulated with
temperature and electric fields.
We are focused on several aspects of microfluidics: (1)
fiber processing,
materials synthesis,
and
pharmaceutical production;
(2) using microscale devices
as a platform to understand
electrokinetic phenomena at interfaces;
and (3)
using microfabricated
structures to culture,
sort, capture
and
study
cells.
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Selected Publications and Presentations on Microfluidic Transport
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Rouillard AD,
Berglund CM,
Lee JY,
Polacheck WJ,
Tsui YT,
Bonassar LJ,
Kirby BJ
"Methods for photocrosslinking alginate hydrogel scaffolds with
high cell viability,"
Tissue Engineering C, accepted, 2010.
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Rouillard AD,
Tsui YT,
Polacheck WJ,
Lee JY,
Bonassar LJ,
Kirby BJ
"Control of the Electromechanical Properties of Alginate Hydrogels via
Ionic and Covalent Crosslinking and Microparticle Doping,"
Biomacromolecules, 11 (8), pp 2184–2189, 2010.
doi
pdf
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Hawkins BG,
Kirby BJ
"Electrothermal flow effects in insulating
(electrodeless) dielectrophoresis systems,"
submitted, 2010.
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Pratt ED,
Huang C,
Hawkins BG,
Gleghorn JP,
Kirby BJ
"Rare cell capture in microfluidic devices, submitted, 2010.
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Rouillard AD,
Berglund CM,
Lee JY,
Polacheck WJ,
Tsui YT,
Bonassar LJ,
Kirby BJ
"Methods for photocrosslinking alginate hydrogel scaffolds with high cell viability,"
Orthopedic Research Society Meeting, New Orleans, LA, March 2010.
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Kirby BJ
"Micro- and Nanoscale Fluid Mechanics: Transport in Microfluidic Devices,"
Cambridge University Press, 2010.
click here for html version|
Cambridge University Press
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Gleghorn JP,
Pratt ED,
Denning D,
Liu H,
Bander NH,
Tagawa S,
Nanus DM,
Giannakakou PA,
Kirby BJ
"Capture of circulating tumor cells from whole blood of
prostate cancer patients using geometrically enhanced
differential immunocapture and a prostate-specific antibody",
Lab on a Chip, 10:27-29, 2010.
doi
pdf
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Tandon V,
Bhagavatula SK,
Kirby BJ
"Disruption of long-term electrokinetic transients in
pressure driven flow via a short-term application of an electric field ",
MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.
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Barbati AC,
Fang C,
Banker GA,
Kirby BJ
"Microfluidic culture devices for observation of axonal organelle transport", ",
MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.
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Gleghorn JP,
Pratt ED,
Lofthus MS,
Levy BE,
Bander NH,
Nanus DM,
Giannakakou PA,
Kirby BJ
"High efficiency capture of circulating tumor cells
from patient blood using geometrically-enhanced differential immunocapture",
MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.
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Kirby BJ
"Nanofluidics" (book review),
Materials Today, 12(5) 51, 2009.
doi
pdf
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Tandon VT,
Bhagavatula S,
Kirby BJ
"Transient Zeta Potential Measurements in Hydrophobic,
TOPAS Microfluidic Substrates,"
Electrophoresis 30(15) 2656-2667, 2009.
doi
pdf
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Kondapalli S,
Kirby BJ
"Refolding of beta-galactosidase: Microfluidic device for reagent metering
and mixing and quantification of refolding yield,"
Microfluidics and Nanofluidics 7(2) 275-281, 2009.
doi
pdf
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Hawkins BG,
Gleghorn JP,
Kirby BJ
"Dielectrophoresis for cell and particle manipulations,"
in Methods in Bioengineering: Biomicrofabrication
and Biomicrofluidics, Ed. J.D. Zahn,
Artech Press, 2009.
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Kondapalli S,
Kirby BJ
"Refolding of beta-galactosidase: Microfluidic device for reagent metering
and mixing and quantification of refolding yield,"
AIChE 2008.
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Kondapalli S,
Kirby BJ
"Microfluidic devices for protein refolding," CHI PepTalk 2008, San Diego, CA, Jan 2008.
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George PA, Hui W, Rana F,
Hawkins BG,
Smith AE,
Kirby BJ
"Integrated microfluidic devices for terahertz spectroscopy of biomolecules",
Optics Express, 16(3) 1577-1582 (2008).
pdf
text
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Hawkins BG,
Smith AE,
Kirby BJ
"High-throughput, continuous-flow, dielectrophoretic
screening of Mycobacterium smegmatis in
coherently patterned, polymeric microchannels",
AIChE 2007, Salt Lake City, UT, November 2007.
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Tandon V,
Bhagavatula SK,
Nelson WC, Sharma AN,
Kirby BJ
"Electrokinetic and scanned probe microscopy characterization
of interfacial nanobubble phenomena in hydrophobic microfluidic
devices", AIChE 2007, Salt Lake City, UT, November 2007.
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Hawkins BG,
Tandon V,
Kirby BJ
"Electrokinetic tools for cellular screening in plastic microdevices: interfacial
characterization and engineering design",
Meeting of the Electrochemical Society, Washington, DC, October 2007.
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Lok SM,
Hinestroza JP,
Kirby BJ
"Spatially resolved microfluidic solvent etching of bicomponent
extrustion nanofibers",
MicroTAS 2007, Paris, France, October 2007.
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Tandon V,
Bhagavatula SK,
Nelson WC,
Kirby BJ
"Zeta potential and electroosmotic mobility in microfluidic devices
fabricated from hydrophobic polymers: 1. The origins of charge",
Electrophoresis 29(5):1092-1101, 2008.
doi
pdf
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Tandon V,
Kirby BJ
"Zeta potential and electroosmotic mobility in microfluidic devices
fabricated from hydrophobic polymers: 2. Slip and interfacial water structure",
Electrophoresis 29(5):1102-1114, 2008.
doi
pdf
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Hawkins BG,
Smith AE,
Kirby BJ
"Towards cellular screening for Mycobacteria: Microchip
monitoring of electrical phenotype",
BMES 2007, Los Angeles, CA, September 2007.
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Tandon V,
Bhagavatula SK,
Nelson WC, Sharma AN,
Kirby BJ
Gordon Research Conference on Microfluidics, Waterville Valley, NH, 2007.
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Kondapalli S,
Putnam DA,
Kirby BJ
Gordon Research Conference on Microfluidics, Waterville Valley, NH, 2007.
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Hawkins BG,
Smith AE,
Syed YA,
Kirby BJ
"Continuous-flow particle separation by 3D insulative
dielectrophoresis using coherently shaped, DC-biased,
AC electric fields,"
Analytical Chemistry, 2007.
doi
pdf
text
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Smith AE,
Hawkins BG,
Syed YA,
Kirby BJ
"Continuous-flow particle sorting at low applied electric fields
using electrodeless dielectrophoresis in ridged polymeric devices,"
MicroTAS 2006 Tokyo, Japan, Nov 2006.
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Smith AE,
Hawkins BG,
Syed YA,
Kirby BJ
"Continuous-flow particle sorting at low applied electric fields
using electrodeless dielectrophoresis in ridged polymeric devices,"
ASME Congress 2006 Chicago, IL, Nov 2006.
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Wilkes JO , with Birmingham SG,
Kirby BJ, Cheng C-Y
"Fluid Mechanics for Chemical Engineers with Microfluidics and CFD,"
Prentice-Hall, 2005.
click here to go to text webpage
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Mela P, van den Berg A, Fintschenko Y,
Cummings EB, Simmons BA,
Kirby BJ
"The zeta potential of cyclo-olefin polymer microchannels and its effects on insulative (electrodeless)
dielectrophoresis particle trapping devices,"
Electrophoresis 26:1792-1799 (2005).
doi
pdf
text
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Reichmuth DS, Shepodd TJ,
Kirby BJ
"Microchip HPLC of peptides and proteins,"
Analytical Chemistry 77:2997-3000 (2005).
doi
pdf
text
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Kirby BJ,
Reichmuth DS, Renzi RF, Shepodd TJ,
Wiedenman BJ
"Microfluidic routing of aqueous and
organic flows at high pressure: Fabrication and
characterization of integrated polymer microvalve elements,"
Lab on a Chip 5:184-190 (2005).
doi
pdf
text
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Reichmuth DS, Shepodd TJ,
Kirby BJ
"On-chip
high-pressure picoliter injector for pressure-driven flow
through porous media," Analytical Chemistry
76:5063-5068 (2004).
doi
pdf
text
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Song S, Singh AK,
Kirby BJ
"Electrophoretic
Concentration of Proteins at Laser-Patterned Porous
Membranes," Analytical Chemistry 76:4589-4592 (2004).
doi
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text |
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Song S, Singh AK, Shepodd TJ,
Kirby BJ
"Microchip dialysis of proteins using in situ
photopatterned nanoporous polymer membranes", Analytical
Chemistry 76:2367-2373 (2004).
doi
pdf
text
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Kirby BJ,
Hasselbrink, Jr. EF
"The Zeta
Potential of Microfluidic Substrates. 1. Theory, experimental
techniques, and effects on separations,"
Electrophoresis, 25:187-202
(2004).
doi
pdf
text
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Kirby BJ,
Hasselbrink, Jr. EF
"The Zeta
Potential of Microfluidic Substrates. 2. Data for polymers,"
Electrophoresis, 25:203-213 (2004).
doi
pdf
text
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Reichmuth DS,
Kirby BJ
"Effects of
Ammonioalkyl sulfonate internal salts on electrokinetic
micropump performance and Reversed-Phase HPLC separations,"
Journal of Chromatography A, 1013:93-101
(2003).
doi
pdf
text
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Reichmuth DS, Chirica GS,
Kirby BJ
"Increasing the Performance of High-Pressure,
High-Efficiency Electrokinetic Micropumps Using Zwitterionic
Solute Additives," Sensors and Actuators B-Chemical, 92:37-43 (2003).
doi
pdf
text
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Fintschenko Y,
Kirby BJ,
Hasselbrink, Jr. EF,
Singh AK, Shepodd TJ
"Monolithic Materials: Miniature
and Microchip Technologies," in Monolithic Materials:
Preparation, Properties, and Applications Elsevier,
Amsterdam (2003).
pdf
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Kirby BJ,
Wheeler AR, Zare RN, Fruetel JA,
Shepodd TJ
"Programmable Modification of Cell Adhesion
and Zeta Potential in Silica Microchips,"Lab On a Chip
3:5-10 (2003).
doi
pdf
text
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Kirby BJ,
Shepodd TJ, Hasselbrink, Jr. EF
"Voltage-Addressable On/Off Microvalves for High-Pressure
Microchip Separations," Journal of Chromatography A
979:147-154 (2002).
doi
pdf
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Left: results showing that geometrically enhanced differential immunocapture can
capture prostate cancer cells (LNCaP) with high efficiency. Right: results showing that
prostate cancer cells can be captured from blood with high purity.
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A protein refolding
microchip with associated external controls. We are developing
microdevices with the goal of accelerating pharmaceutical development.
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