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Microfluidic devices for circulating tumor cell capture
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Funding: NYSTAR, NIH Clinical and Translational Science Center, Prostate Cancer Foundation
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Rare cell capture from blood is exciting owing to the potential
to derive clinical benefit with minimal inconvenience and discomfort to patients.
Information derived from rare cells (e.g., fetal cells in mothers or cancer cells
in cancer patients) can be used in lieu of information from biopsies and thus
improve patient outcomes.
Microfluidic devices are ideally suited for these processes, owing to the
flexibility of geometric design, wealth of chemical manipulation techniques, and
assay compatibility of current systems. Stokes flow analysis is often a good predictor of the flows in these systems.
Our current work, in collaboration with Neil Bander, Evi Giannakakou, and David Nanus
at Weill Cornell Medical Center, is focused on microfluidic capture of circulating
tumor cells from prostate cancer patients with a view towards preclinical evaluation
of chemotherapeutic efficacy.
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Publications and Presentations on Circulating Tumor Cell Capture
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Santana SM,
Liu H,
Bander NH,
Gleghorn JP,
Kirby BJ,
"Immunocapture of Prostate Cancer Cells
with Anti-PSMA Antibodies in Microdevices
", , accepted, 2011.
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Kirby BJ,
Jodari M,
Loftus M,
Pratt ED,
Gakhar G,
Chanel-Vos C,
Gleghorn JP,
Santana SM,
Liu H,
Smith JP,
Bander NH,
Tagawa S,
Nanus DM,
Giannakakou PA,
"Functional Characterization of Circulating Tumor Cells
with a Prostate-Cancer-Specific Microfluidic Device
", submitted, 2011.
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Gleghorn JP,
Smith JP,
Kirby BJ
"Spatial aliasing of particle displacements and collision rates in
periodic asymmetric microfluidic obstacle arrays", submitted, 2011.
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Pratt ED,
Huang C,
Hawkins BG,
Gleghorn JP,
Kirby BJ
"Rare cell capture in microfluidic devices, Chemical Engineering Science, Vol 66(7)1508-1522, 2011.
doi
pdf
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Gleghorn JP,
Pratt ED,
Denning D,
Liu H,
Bander NH,
Tagawa S,
Nanus DM,
Giannakakou PA,
Kirby BJ
"Capture of circulating tumor cells from whole blood of
prostate cancer patients using geometrically enhanced
differential immunocapture and a prostate-specific antibody",
Lab on a Chip, 10:27-29, 2010.
doi
PubMedCentral
pdf
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Gleghorn JP,
Pratt ED,
Lofthus MS,
Levy BE,
Bander NH,
Nanus DM,
Giannakakou PA,
Kirby BJ
"High efficiency capture of circulating tumor cells
from patient blood using geometrically-enhanced differential immunocapture",
MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.
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Left: results showing that geometrically enhanced differential immunocapture can
capture prostate cancer cells (LNCaP) with high efficiency. Right: results showing that
prostate cancer cells can be captured from blood with high purity.
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