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Microfluidic devices for detection of enteric viruses in environmental water
Funding: Environmental Protection Agency

Monitoring the safety of surface water, recreational water, and run-off waters is critical to protect our society from naturally occurring pathogens, as well as unintentional and intentional contaminations. The ability to process large volumes is especially important in environmental analysis, to obtain a sample size that is representative of the water body and to detect pathogens present at very low concentrations (1 pathogen per liter). However, current detection technology does not offer the possibility for rapid on-site analysis of low level pathogen concentrations. Instead, on-site sampling and or filtration is combined with off-site analysis in specialized laboratories.

Conventional methods for the detection of enteric pathogens typically take days and require skilled interpretation, making them unsuitable for rapid response. While these methods have low limits of detection and can be used in difficult sample matrices, their complexity, cost and time precludes their routine and frequent use in environmental sample analysis.

Our current work, in collaboration with Antje Baeumner at Cornell, combines electrokinetic concentration with high-sensitivity immunospecific biosensing to enable detection of very low pathogen concentrations in complex samples.

Archival Publications

PDF version of Connelly Kondapalli Parker Kirby Baeumner: Micro total analysis system for virus detection:microfluidic pre-concentration coupled to liposome-based detection

Connelly JT, Kondapalli S, Skoupi M, Parker JSL, Kirby BJ, Baeumner AJ
"Micro total analysis system for virus detection:microfluidic pre-concentration coupled to liposome-based detection," Analytical and Bioanalytical Chemistry, Volume 402, Number 1, 315-323, 2012. doi pdf

PDF version of Kondapalli Connelly Baeumner Kirby: Integrated microfluidic preconcentrator and immunobiosensor

Kondapalli S, Connelly JT, Baeumner AJ, Kirby BJ
"Integrated microfluidic preconcentrator and immunobiosensor," Microfluidics and Nanofluidics, Volume 11, Number 5, 537-544, 2011. doi pdf

Presentations and Other Publications

20-25 June 2010

Connelly JT, Kondapalli S, Skoupi M, Parker JSL, Kirby BJ, Baeumner AJ
"Electrochemical biosensor with integrated preconcentration module for the detection of enteric viruses," Gordon Research Conference on Bioanalytical Sensors, Colby-Sawyer College, New London, NH.

1-5 Nov 2009

Kondapalli S, Connelly JT, Baeumner AJ, Kirby BJ
"On-chip electrophoretic concentration of liposomes for antibody-based viral biosensors", MicroTAS 2009, Jeju, Korea.

3 Apr 2009

Kondapalli S , Connelly JT, Baeumner AJ, Kirby BJ
"Development and evaluation of pathogen concentrators and microbiosensors for on-site environmental water sampling", Cornell Engineering Research Conference, Ithaca, NY.

27 Oct 2008

Kondapalli S , Kirby BJ
"On-chip electrohporetic Concentration of Liposomes using Photopolymerized Nanoporous Membranes", NBTC Annual Symposium, Ithaca, NY.

21 Mar 2008

Kirby BJ
"Cell processing in microdevices: sorting, capturing, and concentration techniques", University of Rochester Biomedical Engineering Department, Rochester, NY.

Data showing an experimental limit of detection of 2e5 PFU/ml for feline calicivirus in microfluidic devices with liposome concentration and biosensing.
A microdevice for virus detection in environmental water, incorporating laser-patterned membranes.
Antibody functionalization schemes for detection of feline calicivirus.